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Over the past two decades, expressed sequence tags (ESTs - single pass reads from randomly selected cDNAs), have proven to be a remarkably cost-effective route for the purposes of gene discovery. Gaining in popularity, millions of ESTs have now been generated for over a thousand different species. In Expressed Sequence Tags (ESTs): Generation and Analysis, leading experts in the field introduce the reader to many of the fundamental concepts underlying the generation and analysis of ESTs through readily accessible and affordable sequencing technologies. The volume focuses on various methods used to generate, process and analyze EST datasets, while also exploring the use of EST technology for other purposes such as expression profiling, analysis of alternative transcripts, and phylogenomics. Written in the highly successful Methods in Molecular Biology(TM) series format, chapters include brief introductions to their respective topics, lists of the necessary materials and programs, step-by-step, readily reproducible protocols, and Notes sections, which highlight tips on troubleshooting and avoiding known pitfalls. Cutting-edge and easy to use, Expressed Sequence Tags (ESTs): Generation and Analysis serves as an ideal reference for scientists continuing the vital investigation of genes and the genome.

Annie Carter is back...She was a madam in a brothel and a gangster's moll, but now her protection is gone and her enemies are closing in. 1971, London Expressed Sequence Tags (ESTs) : Generation and Analysis download PDF gang boss Annie Carter Barolli is living the New York high life with the feared mafia godfather Constantine Barolli. Then family tragedy strikes, leaving only Annie, Constantine's sister Gina and his three children alive, and now they're in terrible danger. And what's worse - it signals a major shift in mafia power. Annie returns to London with her daughter Layla, pursued by a hit man. Someone wants her dead and the only way she can stay alive is to find out who's paying for the contract and to strike first. Then, the reappearance of an old East End face sparks a shocking suspicion - the possibility that Max Carter, Annie's first and greatest love, didn't die two years ago, as she had been led to believe. Has he truly just been playing dead?

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Author: John Parkinson
Number of Pages: 332 pages
Published Date: 19 Nov 2010
Publisher: Humana Press Inc.
Publication Country: Totowa, NJ, United States
Language: English
ISBN: 9781617377600
Download Link: Click Here
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